DNA Topoisomerase Protocols
Osheroff N., Bjornsti M.A.
DNA Topoisomerase Protocols is an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these time-proven methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, bacterial, yeast, and mammalian expression systems.
巻:
Volume 1
年:
1999
言語:
english
ページ:
317
シリーズ:
Methods in Molecular Biology 094
ファイル:
PDF, 3.87 MB
IPFS:
,
english, 1999
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